4AQV
Model of human kinesin-5 motor domain (3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.7-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the AMPPPNP state.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | TUBULIN ALPHA-1D CHAIN | polymer | 452 | 50236.4 | 1 | UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) In PDB | BOS TAURUS (CATTLE) | ALPHA-TUBULIN |
2 | B | TUBULIN BETA-2B CHAIN | polymer | 445 | 49907.8 | 1 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB | BOS TAURUS (CATTLE) | BETA-TUBULIN |
3 | C | KINESIN-LIKE PROTEIN KIF11 | polymer | 373 | 41673.1 | 1 | UniProt (P52732) Pfam (PF00225) In PDB | HOMO SAPIENS (HUMAN) | KINESIN-5, KINESIN-LIKE PROTEIN 1, KINESIN-LIKE SPINDLE PRO KINESIN-RELATED MOTOR PROTEIN EG5, THYROID RECEPTOR-INTERACTING PROTEIN 5, TR-INTERACTING PROTEIN 5, TRIP-5 |
4 | A, C | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
6 | B | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
7 | B | TAXOL | non-polymer | 853.9 | 1 | Chemie (TA1) | |||
8 | C | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) |
Sequence modifications
A: 1 - 452 (UniProt: Q2HJ86)
B: 1 - 455 (UniProt: Q6B856)
C: 1 - 367 (UniProt: P52732)
PDB | External Database | Details |
---|---|---|
Ile 7 | Val 7 | conflict |
Ile 114 | Leu 114 | conflict |
Ser 136 | Leu 136 | conflict |
Val 137 | Ile 137 | conflict |
Gly 265 | Ile 265 | conflict |
Glu 358 | Gln 358 | conflict |
Val 437 | Met 437 | conflict |
Glu 450 | Asp 450 | conflict |
PDB | External Database | Details |
---|---|---|
Ala 57 | Thr 55 | conflict |
Val 172 | Met 170 | conflict |
Ala 298 | Ser 296 | conflict |
Val 318 | Ile 316 | conflict |
PDB | External Database | Details |
---|---|---|
Val 25 | Cys 25 | engineered mutation |
Ser 43 | Cys 43 | engineered mutation |
Ala 87 | Cys 87 | engineered mutation |
Ala 99 | Cys 99 | engineered mutation |
Cys 126 | Thr 126 | engineered mutation |
His 368 | - | expression tag |
His 369 | - | expression tag |
His 370 | - | expression tag |
His 371 | - | expression tag |
His 372 | - | expression tag |
His 373 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 141817.2 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 2375.1 | |
All* | Total formula weight | 144192.3 |