4AQV

Model of human kinesin-5 motor domain (3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.7-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the AMPPPNP state.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI TECNAI F20
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2
Detector	KODAK SO-163 FILM
Number of Images	46

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	9.7
Software	Flex-EM,UCSF Chimera,FREALIGN,SPIDER