4AQ1

Structure of the SbsB S-layer protein of Geobacillus stearothermophilus PV72p2 in complex with nanobody KB6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SBSB PROTEIN	polymer	892	94961.2	1	UniProt (Q45664) Pfam (PF00395)	GEOBACILLUS STEAROTHERMOPHILUS
2	B, D (B, D)	NBKB6	polymer	130	13992.3	2		LAMA GLAMA (LLAMA)
3	C (C)	SBSB PROTEIN	polymer	892	94975.2	1	UniProt (Q45664) Pfam (PF00395)	GEOBACILLUS STEAROTHERMOPHILUS
4	E, F, G, H, I... (A, C)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
5	M, N, O, P (A, B, C, D)	water	water		18.0	475	Chemie (HOH)

Sequence modifications

A: 32 - 920 (UniProt: Q45664)

PDB	External Database	Details
Met 31	-	expression tag
Gly 86	Asp 86	conflict
Ala 139	Arg 139	conflict
His 140	Tyr 140	conflict
Glu 500	Asp 500	conflict
Glu 812	Lys 812	conflict
Gly 921	-	expression tag
Ser 922	-	expression tag

C: 32 - 920 (UniProt: Q45664)

PDB	External Database	Details
Met 31	-	expression tag
Gly 86	Asp 86	conflict
Ala 139	Arg 139	conflict
His 140	Tyr 140	conflict
Thr 402	Ser 402	conflict
Glu 500	Asp 500	conflict
Glu 812	Lys 812	conflict
Gly 921	-	expression tag
Ser 922	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	217921.0
Non-Polymers*	Number of molecules	8
	Total formula weight	320.6
All*	Total formula weight	218241.7

*Water molecules are not included.