4AQ1
Structure of the SbsB S-layer protein of Geobacillus stearothermophilus PV72p2 in complex with nanobody KB6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-13 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 |
Unit cell lengths | 68.500, 88.500, 95.200 |
Unit cell angles | 112.50, 101.60, 84.70 |
Refinement procedure
Resolution | 43.810 - 2.420 |
R-factor | 0.18565 |
Rwork | 0.183 |
R-free | 0.23567 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.491 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.500 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.110 | 0.650 |
Number of reflections | 74487 | |
<I/σ(I)> | 11 | 2.5 |
Completeness [%] | 97.9 | 97.3 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M KSCN, 0.1 M BIS-TRIS PROPANE PH 6.5 AND 20% PEG 3350 |