4APF

Crystal structure of the human KLHL11-Cul3 complex at 3.1A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	KELCH-LIKE PROTEIN 11	polymer	297	34526.2	1	UniProt (Q9NVR0) Pfam (PF00651) Pfam (PF07707) In PDB	HOMO SAPIENS (HUMAN)
2	B	CULLIN 3	polymer	388	45724.0	1	UniProt (Q13618) Pfam (PF00888) In PDB	HOMO SAPIENS (HUMAN)	CUL-3
3		water	water		18.0	10	Chemie (HOH)

Sequence modifications

A: 67 - 340 (UniProt: Q9NVR0)

PDB	External Database	Details
Met 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
Ser 51	-	expression tag
Ser 52	-	expression tag
Gly 53	-	expression tag
Val 54	-	expression tag
Asp 55	-	expression tag
Leu 56	-	expression tag
Gly 57	-	expression tag
Thr 58	-	expression tag
Glu 59	-	expression tag
Asn 60	-	expression tag
Leu 61	-	expression tag
Tyr 62	-	expression tag
Phe 63	-	expression tag
Gln 64	-	expression tag
Ser 65	-	expression tag
Met 66	-	expression tag

B: 23 - 388 (UniProt: Q13618)

PDB	External Database	Details
Ala 389	-	expression tag
Glu 390	-	expression tag
Asn 391	-	expression tag
Leu 392	-	expression tag
Tyr 393	-	expression tag
Phe 394	-	expression tag
Gln 395	-	expression tag
Ser 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
Asp 403	-	expression tag
Tyr 404	-	expression tag
Lys 405	-	expression tag
Asp 406	-	expression tag
Asp 407	-	expression tag
Asp 408	-	expression tag
Asp 409	-	expression tag
Lys 410	-	expression tag
Arg 342	Ile 342	engineered mutation
Asp 346	Leu 346	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80250.2
All*	Total formula weight	80250.2

*Water molecules are not included.