4AP4

Rnf4 - ubch5a - ubiquitin heterotrimeric complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 UBIQUITIN LIGASE RNF4	polymer	133	14758.2	1	UniProt (O88846) Pfam (PF13639) In PDB	RATTUS NORVEGICUS (NORWAY RAT)	RING FINGER PROTEIN 4, SMALL NUCLEAR RING FINGER PROTEIN, PROTEIN SNURF
2	B, E	UBIQUITIN-CONJUGATING ENZYME E2 D1	polymer	153	17134.6	2	UniProt (P51668) Pfam (PF00179) In PDB	HOMO SAPIENS (HUMAN)	UBCH5A, STIMULATOR OF FE TRANSPORT, SFT, UBC4/5 HOMOLOG, UBCH5, UBIQUITIN CARRIER PROTEIN D1, UBIQUITIN-CONJUGATING ENZYME E2(17)KB 1, UBIQUITIN-CONJUGATING ENZYME E2-17 KDA 1, UBIQUITIN-PROTEIN LIGASE D1
3	C, F	UBIQUITIN C	polymer	80	8893.2	2	UniProt (F5H7Y5) Pfam (PF00240) UniProt (by SIFTS) (P0CG48) In PDB	HOMO SAPIENS (HUMAN)
4	A	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5		water	water		18.0	137	Chemie (HOH)

Sequence modifications

A: 131 - 194 (UniProt: O88846)

PDB	External Database	Details
Gly 127	-	expression tag
Ala 128	-	expression tag
Met 129	-	expression tag
Gly 130	-	expression tag

A: 196 - 259 (UniProt: O88846)

PDB	External Database	Details
Gly 195	-	linker

B, E: 1 - 147 (UniProt: P51668)

PDB	External Database	Details
Gly -5	-	expression tag
Ala -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

C, F: 0 - 76 (UniProt: F5H7Y5)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	66813.7
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	67075.3

*Water molecules are not included.