4AMI

Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist bucindolol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	BETA-1 ADRENERGIC RECEPTOR	polymer	315	35940.8	2	UniProt (P07700) Pfam (PF00001)	MELEAGRIS GALLOPAVO (TURKEY)	BETA-1 ADRENORECEPTOR, BETA-1 ADRENOCEPTOR, BETA-T
2	C, G (A, B)	2-[(2S)-3-[[1-(1H-indol-3-yl)-2-methyl-propan-2-yl]amino]-2-oxidanyl-propoxy]benzenecarbonitrile	non-polymer		363.5	2	Chemie (G90)
3	D, E, F, H, I (A, B)	HEGA-10	non-polymer		379.5	5	Chemie (2CV)
4	J, K (A, B)	water	water		18.0	13	Chemie (HOH)

Sequence modifications

A, B: 33 - 243 (UniProt: P07700)

PDB	External Database	Details
Met 31	-	expression tag
Gly 32	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
Ser 68	Arg 68	engineered mutation
Val 90	Met 90	engineered mutation
Leu 116	Cys 116	engineered mutation
Ala 227	Tyr 227	engineered mutation

A, B: 272 - 368 (UniProt: P07700)

PDB	External Database	Details
Leu 282	Ala 282	engineered mutation
Ala 327	Phe 327	engineered mutation
Met 338	Phe 338	engineered mutation
Ala 358	Cys 358	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71881.6
Non-Polymers*	Number of molecules	7
	Total formula weight	2624.4
All*	Total formula weight	74505.9

*Water molecules are not included.