4AMI
Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist bucindolol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.844, 60.677, 107.818 |
| Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
| Resolution | 44.820 - 3.200 |
| R-factor | 0.24421 |
| Rwork | 0.242 |
| R-free | 0.27892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y02 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.984 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.100 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 17466 | |
| <I/σ(I)> | 9.9 | 2.5 |
| Completeness [%] | 95.9 | 86.5 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | 0.1 M BICINE PH 9.0, 22% PEG 600, 4 DEGREES CELSIUS. |
