4AMI
Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist bucindolol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-03 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.844, 60.677, 107.818 |
Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
Resolution | 44.820 - 3.200 |
R-factor | 0.24421 |
Rwork | 0.242 |
R-free | 0.27892 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y02 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.984 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.100 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.100 | 0.600 |
Number of reflections | 17466 | |
<I/σ(I)> | 9.9 | 2.5 |
Completeness [%] | 95.9 | 86.5 |
Redundancy | 5.3 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 277 | 0.1 M BICINE PH 9.0, 22% PEG 600, 4 DEGREES CELSIUS. |