4AM8

Crystal structure of the R54G mutant of putrescine transcarbamylase from Enterococcus faecalis bound to a curing guanidinium ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	PUTRESCINE CARBAMOYLTRANSFERASE	polymer	355	40041.3	6	UniProt (Q837U7) Pfam (PF02729) Pfam (PF00185) In PDB	ENTEROCOCCUS FAECALIS	PTC, PTCASE, AGMATINE CATABOLISM PROTEIN B, PUTRESCINE TRANSCARBAMOYLASE, PUTRESCINE TRANSCARBAMYLASE
2	A, C, E	GLUTAMINE	non-polymer		146.1	3	Chemie (GLN)
3	F, A, B, C, D...	N-(PHOSPHONOACETYL)-L-ORNITHINE	non-polymer		254.2	6	Chemie (PAO)
4	F, A, B, C, D...	GUANIDINE	non-polymer		59.1	7	Chemie (GAI)
5	F, A, B, C	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	4	Chemie (TRS)
6	A, F	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
7	B	ISOLEUCINE	non-polymer		131.2	1	Chemie (ILE)
8		water	water		18.0	825	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 339 (UniProt: Q837U7)

PDB	External Database	Details
Ser 340	-	expression tag
Ala 341	-	expression tag
Ala 342	-	expression tag
Lys 343	-	expression tag
Leu 344	-	expression tag
Ala 345	-	expression tag
Ala 346	-	expression tag
Ala 347	-	expression tag
Leu 348	-	expression tag
Glu 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
Gly 54	Arg 54	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	240247.5
Non-Polymers*	Number of molecules	23
	Total formula weight	3114.1
All*	Total formula weight	243361.6

*Water molecules are not included.