4AM8
Crystal structure of the R54G mutant of putrescine transcarbamylase from Enterococcus faecalis bound to a curing guanidinium ion
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | PUTRESCINE CARBAMOYLTRANSFERASE | polymer | 355 | 40041.3 | 6 | UniProt (Q837U7) Pfam (PF02729) Pfam (PF00185) In PDB | ENTEROCOCCUS FAECALIS | PTC, PTCASE, AGMATINE CATABOLISM PROTEIN B, PUTRESCINE TRANSCARBAMOYLASE, PUTRESCINE TRANSCARBAMYLASE |
2 | A, C, E | GLUTAMINE | non-polymer | 146.1 | 3 | Chemie (GLN) | |||
3 | F, A, B, C, D... | N-(PHOSPHONOACETYL)-L-ORNITHINE | non-polymer | 254.2 | 6 | Chemie (PAO) | |||
4 | F, A, B, C, D... | GUANIDINE | non-polymer | 59.1 | 7 | Chemie (GAI) | |||
5 | F, A, B, C | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 4 | Chemie (TRS) | |||
6 | A, F | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
7 | B | ISOLEUCINE | non-polymer | 131.2 | 1 | Chemie (ILE) | |||
8 | water | water | 18.0 | 825 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 339 (UniProt: Q837U7)
PDB | External Database | Details |
---|---|---|
Ser 340 | - | expression tag |
Ala 341 | - | expression tag |
Ala 342 | - | expression tag |
Lys 343 | - | expression tag |
Leu 344 | - | expression tag |
Ala 345 | - | expression tag |
Ala 346 | - | expression tag |
Ala 347 | - | expression tag |
Leu 348 | - | expression tag |
Glu 349 | - | expression tag |
His 350 | - | expression tag |
His 351 | - | expression tag |
His 352 | - | expression tag |
His 353 | - | expression tag |
His 354 | - | expression tag |
His 355 | - | expression tag |
Gly 54 | Arg 54 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 240247.5 | |
Non-Polymers* | Number of molecules | 23 |
Total formula weight | 3114.1 | |
All* | Total formula weight | 243361.6 |