Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4AM8

Crystal structure of the R54G mutant of putrescine transcarbamylase from Enterococcus faecalis bound to a curing guanidinium ion

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004585	molecular_function	ornithine carbamoyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0006520	biological_process	amino acid metabolic process
A	0006596	biological_process	polyamine biosynthetic process
A	0016597	molecular_function	amino acid binding
A	0016740	molecular_function	transferase activity
A	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
A	0019240	biological_process	citrulline biosynthetic process
A	0033388	biological_process	putrescine biosynthetic process from arginine
A	0033390	biological_process	putrescine biosynthetic process from arginine via N-carbamoylputrescine
A	0042450	biological_process	arginine biosynthetic process via ornithine
A	0050231	molecular_function	putrescine carbamoyltransferase activity
B	0004585	molecular_function	ornithine carbamoyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0006520	biological_process	amino acid metabolic process
B	0006596	biological_process	polyamine biosynthetic process
B	0016597	molecular_function	amino acid binding
B	0016740	molecular_function	transferase activity
B	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
B	0019240	biological_process	citrulline biosynthetic process
B	0033388	biological_process	putrescine biosynthetic process from arginine
B	0033390	biological_process	putrescine biosynthetic process from arginine via N-carbamoylputrescine
B	0042450	biological_process	arginine biosynthetic process via ornithine
B	0050231	molecular_function	putrescine carbamoyltransferase activity
C	0004585	molecular_function	ornithine carbamoyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0006520	biological_process	amino acid metabolic process
C	0006596	biological_process	polyamine biosynthetic process
C	0016597	molecular_function	amino acid binding
C	0016740	molecular_function	transferase activity
C	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
C	0019240	biological_process	citrulline biosynthetic process
C	0033388	biological_process	putrescine biosynthetic process from arginine
C	0033390	biological_process	putrescine biosynthetic process from arginine via N-carbamoylputrescine
C	0042450	biological_process	arginine biosynthetic process via ornithine
C	0050231	molecular_function	putrescine carbamoyltransferase activity
D	0004585	molecular_function	ornithine carbamoyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0006520	biological_process	amino acid metabolic process
D	0006596	biological_process	polyamine biosynthetic process
D	0016597	molecular_function	amino acid binding
D	0016740	molecular_function	transferase activity
D	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
D	0019240	biological_process	citrulline biosynthetic process
D	0033388	biological_process	putrescine biosynthetic process from arginine
D	0033390	biological_process	putrescine biosynthetic process from arginine via N-carbamoylputrescine
D	0042450	biological_process	arginine biosynthetic process via ornithine
D	0050231	molecular_function	putrescine carbamoyltransferase activity
E	0004585	molecular_function	ornithine carbamoyltransferase activity
E	0005737	cellular_component	cytoplasm
E	0006520	biological_process	amino acid metabolic process
E	0006596	biological_process	polyamine biosynthetic process
E	0016597	molecular_function	amino acid binding
E	0016740	molecular_function	transferase activity
E	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
E	0019240	biological_process	citrulline biosynthetic process
E	0033388	biological_process	putrescine biosynthetic process from arginine
E	0033390	biological_process	putrescine biosynthetic process from arginine via N-carbamoylputrescine
E	0042450	biological_process	arginine biosynthetic process via ornithine
E	0050231	molecular_function	putrescine carbamoyltransferase activity
F	0004585	molecular_function	ornithine carbamoyltransferase activity
F	0005737	cellular_component	cytoplasm
F	0006520	biological_process	amino acid metabolic process
F	0006596	biological_process	polyamine biosynthetic process
F	0016597	molecular_function	amino acid binding
F	0016740	molecular_function	transferase activity
F	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
F	0019240	biological_process	citrulline biosynthetic process
F	0033388	biological_process	putrescine biosynthetic process from arginine
F	0033390	biological_process	putrescine biosynthetic process from arginine via N-carbamoylputrescine
F	0042450	biological_process	arginine biosynthetic process via ornithine
F	0050231	molecular_function	putrescine carbamoyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE PAO A 1338

Chain	Residue
A	SER52
A	LEU270
A	ARG297
A	GAI403
A	HOH524
A	HOH550
A	HOH626
A	HOH590
A	HOH509
A	HOH583
A	THR53
A	GLY54
A	THR55
A	ARG103
A	HIS130
A	THR163
A	GLN164
A	ASP227

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GAI A 1339

Chain	Residue
A	GLN50
A	SER51
A	ARG103
A	MET125
A	PAO402
A	HOH509

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE TRS A 1340

Chain	Residue
A	ASP161
A	ALA162
A	PHE188
A	HOH555

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NI A 1341

Chain	Residue
A	HIS69
A	HOH563
C	HOH540
B	HIS69
B	HOH531
C	HIS69

site_id	AC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE PAO B 1340

Chain	Residue
B	SER52
B	THR53
B	GLY54
B	THR55
B	ARG103
B	HIS130
B	THR163
B	GLN164
B	ASP227
B	LEU270
B	ARG297
B	GAI403
B	HOH598
B	HOH555
B	HOH553
B	HOH622
B	HOH605
B	HOH570
B	HOH518

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GAI B 1341

Chain	Residue
B	GLN50
B	SER51
B	ARG103
B	MET125
B	TYR233
B	PAO402
B	HOH605
B	HOH518

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE TRS B 1342

Chain	Residue
B	ASP161
B	ALA162
B	PHE188
B	GLY231
B	LEU232
B	HOH544
B	HOH504
B	HOH502
B	HOH558

site_id	AC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE PAO C 1338

Chain	Residue
C	SER52
C	THR53
C	GLY54
C	THR55
C	ARG103
C	HIS130
C	GLN164
C	ASP227
C	LEU270
C	ARG297
C	GAI403
C	HOH606
C	HOH539
C	HOH619
C	HOH591
C	HOH615

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GAI C 1339

Chain	Residue
C	GLN50
C	SER51
C	ARG103
C	MET125
C	TYR233
C	PAO402

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE TRS C 1340

Chain	Residue
C	ASP161
C	ALA162
C	GLY231
C	HOH521
C	HOH575

site_id	BC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE PAO D 1350

Chain	Residue
D	GLY54
D	THR55
D	ARG103
D	HIS130
D	THR163
D	GLN164
D	VAL165
D	ASP227
D	LEU270
D	ARG297
D	GAI402
D	HOH513
D	HOH640
D	HOH600
D	HOH561
D	HOH569
D	SER52
D	THR53

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GAI D 1351

Chain	Residue
D	GLN50
D	SER51
D	ARG103
D	MET125
D	PAO401
D	HOH569

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NI D 1352

Chain	Residue
D	HIS69
D	HOH510
F	HOH537
E	HIS69
E	HOH543
F	HIS69

site_id	BC5
Number of Residues	15
Details	BINDING SITE FOR RESIDUE PAO E 1338

Chain	Residue
E	SER52
E	THR53
E	GLY54
E	THR55
E	ARG103
E	HIS130
E	GLN164
E	ASP227
E	LEU270
E	ARG297
E	GAI403
E	HOH563
E	HOH548
E	HOH541
E	HOH561

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GAI E 1339

Chain	Residue
E	GLN50
E	SER51
E	ARG103
E	MET125
E	TYR233
E	PAO402

site_id	BC7
Number of Residues	19
Details	BINDING SITE FOR RESIDUE PAO F 1337

Chain	Residue
F	SER52
F	THR53
F	GLY54
F	THR55
F	ARG103
F	HIS130
F	GLN164
F	VAL165
F	ASP227
F	LEU270
F	ARG297
D	GAI403
F	GAI403
F	HOH556
F	HOH583
F	HOH544
F	HOH577
F	HOH511
D	HOH555

site_id	BC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GAI F 1338

Chain	Residue
D	GLU84
D	VAL92
F	GLY54
F	PRO271
F	PAO402
D	HOH614
D	HOH555

site_id	BC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GAI F 1339

Chain	Residue
F	GLN50
F	SER51
F	ARG103
F	MET125
F	TYR233
F	PAO402
F	HOH577
F	HOH511

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE TRS F 1340

Chain	Residue
F	ASP161
F	ALA162
F	PHE188
F	LEU232

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
D	SER52
D	ARG103
D	HIS130
D	HIS268
E	SER52
E	ARG103
E	HIS130
E	HIS268
F	SER52
F	ARG103
F	HIS130
F	HIS268
A	SER52
A	ARG103
A	HIS130
A	HIS268
B	SER52
B	ARG103
B	HIS130
B	HIS268
C	SER52
C	ARG103
C	HIS130
C	HIS268

site_id	SWS_FT_FI2
Number of Residues	12
Details	SITE: Important for structural integrity => ECO:0000250

Chain	Residue	Details
A	LYS27
A	GLU143
B	LYS27
B	GLU143
C	LYS27
C	GLU143
D	LYS27
D	GLU143
E	LYS27
E	GLU143
F	LYS27
F	GLU143

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)