4AHC
Crystal Structure of an Evolved Replicating DNA Polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA POLYMERASE | polymer | 775 | 90113.2 | 2 | UniProt (P61875) Pfam (PF03104) Pfam (PF00136) | PYROCOCCUS FURIOSUS | PFU POLYMERASE |
| 2 | C, D, E, F (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 3 | G, H (A, B) | water | water | 18.0 | 294 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 775 (UniProt: P61875)
| PDB | External Database | Details |
|---|---|---|
| Gln 93 | Val 93 | engineered mutation |
| Ala 141 | Asp 141 | engineered mutation |
| Ala 143 | Glu 143 | engineered mutation |
| Ile 337 | Val 337 | engineered mutation |
| Asp 399 | Glu 399 | engineered mutation |
| Asp 400 | Asn 400 | engineered mutation |
| Ile 407 | Arg 407 | engineered mutation |
| His 546 | Tyr 546 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 180226.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 368.4 | |
| All* | Total formula weight | 180594.7 |
