4AHC
Crystal Structure of an Evolved Replicating DNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-30 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.438, 197.862, 78.438 |
| Unit cell angles | 90.00, 108.39, 90.00 |
Refinement procedure
| Resolution | 98.950 - 2.400 |
| R-factor | 0.20739 |
| Rwork | 0.206 |
| R-free | 0.22936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.761 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 78466 | |
| <I/σ(I)> | 9.6 | 2.1 |
| Completeness [%] | 89.1 | 83 |
| Redundancy | 2.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.08M SODIUM CACODYLATE PH 6.5 0.14M MAGNESIUM ACETATE 12% PEG 8K 20% GLYCEROL |
