4AF8

The structural basis for metacaspase substrate specificity and activation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	METACASPASE MCA2	polymer	367	39972.3	1	UniProt (Q585F3) Pfam (PF00656) In PDB	TRYPANOSOMA BRUCEI
2	A	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4		water	water		18.0	292	Chemie (HOH)

Sequence modifications

A: 1 - 347 (UniProt: Q585F3)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 106	Lys 106	SEE REMARK 999
Gly 213	Cys 213	engineered mutation
Arg 315	Gly 315	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39972.3
Non-Polymers*	Number of molecules	6
	Total formula weight	162.9
All*	Total formula weight	40135.2

*Water molecules are not included.