4AE4

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a novel SOUBA domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	UBIQUITIN-ASSOCIATED PROTEIN 1	polymer	118	13800.4	1	UniProt (Q9NZ09) Pfam (PF22567) Pfam (PF21267)	HOMO SAPIENS (HUMAN)	UBAP-1, UBAP1, NASOPHARYNGEAL CARCINOMA-ASSOCIATED GENE 20 PROTEIN
2	B (B)	UBIQUITIN-ASSOCIATED PROTEIN 1	polymer	118	13832.4	1	UniProt (Q9NZ09) Pfam (PF22567) Pfam (PF21267)	HOMO SAPIENS (HUMAN)	UBAP-1, UBAP1, NASOPHARYNGEAL CARCINOMA-ASSOCIATED GENE 20 PROTEIN
3	C, G (A, B)	2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID	non-polymer		207.3	2	Chemie (NHE)
4	D, F, H, I (A, B)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
5	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	J (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	K, L (A, B)	water	water		18.0	242	Chemie (HOH)

Sequence modifications

A: 389 - 502 (UniProt: Q9NZ09)

PDB	External Database	Details
Gly 385	-	expression tag
Ser 386	-	expression tag
His 387	-	expression tag
Mse 388	-	expression tag
Ala 415	Lys 415	engineered mutation
Ala 416	Lys 416	engineered mutation
Ala 418	Glu 418	engineered mutation

B: 389 - 502 (UniProt: Q9NZ09)

PDB	External Database	Details
Gly 385	-	expression tag
Ser 386	-	expression tag
His 387	-	expression tag
Mse 388	-	expression tag
Ala 415	Lys 415	engineered mutation
Ala 416	Lys 416	engineered mutation
Ala 418	Glu 418	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27632.8
Non-Polymers*	Number of molecules	8
	Total formula weight	686.1
All*	Total formula weight	28318.8

*Water molecules are not included.