4AE4
The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a novel SOUBA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-24 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.404, 43.480, 59.413 |
| Unit cell angles | 102.58, 96.47, 113.24 |
Refinement procedure
| Resolution | 28.274 - 1.650 |
| R-factor | 0.1516 |
| Rwork | 0.149 |
| R-free | 0.19070 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.536 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE (via) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 36253 | |
| <I/σ(I)> | 9.9 | 1.9 |
| Completeness [%] | 95.9 | 94.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 645 MM NA/K TARTRATE, 271 MM LISO4, 100 MM CHES PH 9.5, 2 MM TCEP, 5% GLYCEROL |
