4A0L

Structure of DDB1-DDB2-CUL4B-RBX1 bound to a 12 bp abasic site containing DNA-duplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	DNA DAMAGE-BINDING PROTEIN 1	polymer	1144	127425.8	2	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178) In PDB	HOMO SAPIENS (HUMAN)	DDB P127 SUBUNIT, DNA DAMAGE-BINDING PROTEIN A, DDBA, DAMAGE-SPECIFIC DNA-BINDING PROTEIN 1, HBV X-ASSOCIATED PROTEIN 1, XAP-1, UV-DAMAGED DNA-BINDING FACTOR, UV-DAMAGED DNA-BINDING PROTEIN 1, UV-DDB 1, XPE-BINDING FACTOR, XPE-BF, XERODERMA PIGMENTOSUM GROUP E-COMPLEMENTING PROTEIN, XPCE
2	B, D	DNA DAMAGE-BINDING PROTEIN 2	polymer	382	43418.1	2	UniProt (Q2YDS1) Pfam (PF00400) In PDB	DANIO RERIO (ZEBRAFISH)	DAMAGE-SPECIFIC DNA-BINDING PROTEIN 2
3	E, H	CULLIN-4B	polymer	726	84992.2	2	UniProt (Q13620) Pfam (PF00888) Pfam (PF10557) In PDB	HOMO SAPIENS (HUMAN)	CULLIN HOMOLOG 4B, CUL-4B
4	F, I	E3 UBIQUITIN-PROTEIN LIGASE RBX1	polymer	98	11330.9	2	UniProt (P62878) Pfam (PF12678) In PDB	MUS MUSCULUS (HOUSE MOUSE)	RING FINGER PROTEIN 75, RING-BOX PROTEIN 1, RBX1
5	R, T	12 BP THF CONTAINING DNA DUPLEX	polymer	12	3498.3	2		SYNTHETIC CONSTRUCT
6	S, U	12 BP DNA DUPLEX	polymer	12	3702.4	2		SYNTHETIC CONSTRUCT

Sequence modifications

A, C: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

B, D: 94 - 457 (UniProt: Q2YDS1)

PDB	External Database	Details
Met 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
Arg 83	-	expression tag
Arg 84	-	expression tag
Leu 85	-	expression tag
Val 86	-	expression tag
Pro 87	-	expression tag
Arg 88	-	expression tag
Gly 89	-	expression tag
Ser 90	-	expression tag
Gly 91	-	expression tag
Gly 92	-	expression tag
Arg 93	-	expression tag
Gln 180	Leu 146	variant
Arg 214	Trp 180	variant

E, H: 193 - 913 (UniProt: Q13620)

PDB	External Database	Details
Gly 189	-	expression tag
Gly 190	-	expression tag
Gly 191	-	expression tag
Arg 192	-	expression tag

F, I: 12 - 108 (UniProt: P62878)

PDB	External Database	Details
Met 11	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	548735.5
All*	Total formula weight	548735.5

*Water molecules are not included.