4A0L
Structure of DDB1-DDB2-CUL4B-RBX1 bound to a 12 bp abasic site containing DNA-duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.800, 155.840, 255.390 |
| Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
| Resolution | 29.862 - 7.400 |
| R-factor | 0.318 |
| Rwork | 0.318 |
| R-free | 0.31990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3EI2 4a0c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 7.590 |
| High resolution limit [Å] | 7.400 | 7.400 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 13547 | |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 97.5 | 99.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 100MM MES PH 6.2, 3.1% PEG 6000, 4% ETHYLENEGLYCOL |
