45IM

Cryo-EM structure of the outwardly rectifying potassium channel TOK1 from Saccharomyces cerevisiae in a lipid nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Outward-rectifier potassium channel TOK1	polymer	758	84432.7	2	UniProt (P40310) Pfam (PF07885)	Saccharomyces cerevisiae (brewer's yeast)	Two-domain outward rectifier K(+) channel YORK
2	C, D, E, K, L... (A, B)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	6	Chemie (Y01) PubChem (65082)
3	F, G, H, I (A)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K) PubChem (813)
4	J, N (A, B)	1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine	non-polymer		760.1	2	Chemie (LBN)

Sequence modifications

A, B: 2 - 691 (UniProt: P40310)

PDB	External Database	Details
Met -34	-	initiating methionine
Ser -33	-	expression tag
Ala -32	-	expression tag
Trp -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
Pro -28	-	expression tag
Gln -27	-	expression tag
Phe -26	-	expression tag
Glu -25	-	expression tag
Lys -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Gly -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Ala -12	-	expression tag
Trp -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
Pro -8	-	expression tag
Gln -7	-	expression tag
Phe -6	-	expression tag
Glu -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 692	-	expression tag
Gly 693	-	expression tag
Ser 694	-	expression tag
Gly 695	-	expression tag
Gly 696	-	expression tag
Ser 697	-	expression tag
Gly 698	-	expression tag
Pro 699	-	expression tag
Ser 700	-	expression tag
Arg 701	-	expression tag
Leu 702	-	expression tag
Glu 703	-	expression tag
Glu 704	-	expression tag
Glu 705	-	expression tag
Leu 706	-	expression tag
Arg 707	-	expression tag
Arg 708	-	expression tag
Arg 709	-	expression tag
Leu 710	-	expression tag
Thr 711	-	expression tag
Glu 712	-	expression tag
Pro 713	-	expression tag
Gly 714	-	expression tag
Ser 715	-	expression tag
His 716	-	expression tag
His 717	-	expression tag
His 718	-	expression tag
His 719	-	expression tag
His 720	-	expression tag
His 721	-	expression tag
His 722	-	expression tag
His 723	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	168865.4
Non-Polymers*	Number of molecules	12
	Total formula weight	4596.9
All*	Total formula weight	173462.3

*Water molecules are not included.