3ZUV

Crystal structure of a designed selected Ankyrin Repeat protein in complex with the phosphorylated MAP kinase ERK2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	MITOGEN-ACTIVATED PROTEIN KINASE 1	polymer	364	42319.4	2	UniProt (P63086) Pfam (PF00069)	RATTUS NORVEGICUS (NORWAY RAT)	MAP KINASE 1, MAPK 1, ERT1, EXTRACELLULAR SIGNAL-REGULATED KINASE 2, ERK-2, MAP KINASE ISOFORM P42, P42-MAPK, MITOGEN-ACTIVATED PROTEIN KINASE 2, MAP KINASE 2, MAPK 2
2	B, D (B, D)	DESIGNED ANKYRIN REPEAT PROTEIN	polymer	136	14749.5	2		SYNTHETIC CONSTRUCT
3	E, F (A, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	G, H, I, J (A, B, C, D)	water	water		18.0	203	Chemie (HOH)

Sequence modifications

A, C: 3 - 358 (UniProt: P63086)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 1	-	expression tag
Met 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	114137.8
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	114330.0

*Water molecules are not included.