3ZLF

Structure of group A Streptococcal enolase K312A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	ENOLASE	polymer	455	49518.5	4	UniProt (Q5XD01) Pfam (PF03952) Pfam (PF00113)	STREPTOCOCCUS PYOGENES MGAS10394	2-PHOSPHO-D-GLYCERATE HYDRO-LYASE, 2-PHOSPHOGLYCERATE DEHYDRATASE
2	E, F, G, H, I... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
3	M, N, O, P (A, B, C, D)	water	water		18.0	577	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 435 (UniProt: Q5XD01)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 312	Lys 312	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	198073.9
Non-Polymers*	Number of molecules	8
	Total formula weight	759.8
All*	Total formula weight	198833.6

*Water molecules are not included.