3ZLF

Structure of group A Streptococcal enolase K312A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-31
Detector	ADSC QUANTUM 210r
Spacegroup name	P 4
Unit cell lengths	181.620, 181.620, 56.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.810 - 2.150
R-factor	0.1763
Rwork	0.172
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w6t
RMSD bond length	0.005
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.810	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.240	1.210
Number of reflections	100879
<I/σ(I)>	10.64	2.13
Completeness [%]	100.0	100
Redundancy	11.8	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		1-3.5 M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 5-7.5), 2% PEG 400