3ZF6

Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase D81A D110C S168C mutant with dUpNHpp).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DUTPASE	polymer	204	22627.7	1	UniProt (A4ZF98) Pfam (PF00692) In PDB	STAPHYLOCOCCUS PHAGE 80ALPHA
2	A	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
3	A	2'-DEOXYURIDINE 5'-MONOPHOSPHATE	non-polymer		308.2	1	Chemie (UMP)
4		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A: 1 - 170 (UniProt: A4ZF98)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 81	Asp 81	engineered mutation
Cys 110	Asp 110	engineered mutation
Cys 168	Ser 168	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22627.7
Non-Polymers*	Number of molecules	3
	Total formula weight	425.6
All*	Total formula weight	23053.3

*Water molecules are not included.