3ZF6
Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase D81A D110C S168C mutant with dUpNHpp).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 87.460, 87.460, 87.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.710 - 2.600 |
| R-factor | 0.22758 |
| Rwork | 0.224 |
| R-free | 0.27328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zez |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.869 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.110 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.400 |
| Number of reflections | 7102 | |
| <I/σ(I)> | 23.2 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-8% TERT-BUTANOL, 0.1M TRID (PH 8.5). 30-50% MPD OR PEG400. |
