3ZF5

Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase Y84F mutant with dUpNHpp).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DUTPASE	polymer	204	22651.6	1	UniProt (A4ZF98) Pfam (PF00692)	STAPHYLOCOCCUS PHAGE 80ALPHA
2	B (A)	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DUP)
3	C, D (A)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
4	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	F (A)	water	water		18.0	31	Chemie (HOH)

Sequence modifications

A: 1 - 170 (UniProt: A4ZF98)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Phe 84	Tyr 84	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22651.6
Non-Polymers*	Number of molecules	4
	Total formula weight	608.8
All*	Total formula weight	23260.5

*Water molecules are not included.