3ZEV

Structure of Thermostable Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NEUROTENSIN RECEPTOR 1 TM86V	polymer	338	37607.1	2	UniProt (P20789) Pfam (PF00001)	RATTUS NORVEGICUS (NORWAY RAT)
2	C, D (C, D)	NEUROTENSIN	polymer	8	933.1	2	UniProt (P20068)	RATTUS NORVEGICUS (NORWAY RAT)
3	E, F, G, H, I (A)	GLYCINE	non-polymer		75.1	5	Chemie (GLY)

Sequence modifications

A, B: 50 - 390 (UniProt: P20789)

PDB	External Database	Details
Gly 46	-	expression tag
Pro 47	-	expression tag
Gly 48	-	expression tag
Ser 49	-	expression tag
Thr 391	-	expression tag
Arg 392	-	expression tag
Glu 393	-	expression tag
Leu 394	-	expression tag
Glu 395	-	expression tag
Val 396	-	expression tag
Leu 397	-	expression tag
Phe 398	-	expression tag
Gln 399	-	expression tag
Leu 86	Ala 86	engineered mutation
Asp 103	His 103	engineered mutation
Tyr 105	His 105	engineered mutation
Val 161	Ala 161	engineered mutation
Leu 167	Arg 167	engineered mutation
Leu 213	Arg 213	engineered mutation
Leu 234	Val 234	engineered mutation
Ala 253	Ile 253	engineered mutation
-	Val 280	deletion
-	Gly 281	deletion
-	Thr 282	deletion
-	His 283	deletion
-	Asn 284	deletion
-	Gly 285	deletion
-	Leu 286	deletion
-	Glu 287	deletion
-	His 288	deletion
-	Ser 289	deletion
-	Thr 290	deletion
-	Phe 291	deletion
-	Asn 292	deletion
-	Met 293	deletion
-	Thr 294	deletion
-	Ile 295	deletion
-	Thr 296	deletion
Arg 305	His 305	engineered mutation
Val 358	Phe 358	engineered mutation
Ala 362	Ser 362	engineered mutation

C, D: 8 - 13 (UniProt: P20068)

PDB	External Database	Details
Gly 6	-	expression tag
Gly 7	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	77080.4
Non-Polymers*	Number of molecules	5
	Total formula weight	375.3
All*	Total formula weight	77455.8

*Water molecules are not included.