3ZEV
Structure of Thermostable Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.550, 90.180, 209.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.935 - 3.000 |
R-factor | 0.2436 |
Rwork | 0.242 |
R-free | 0.27920 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.735 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.010 |
Number of reflections | 22942 |
<I/σ(I)> | 8.68 |
Completeness [%] | 99.8 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9.4 | 1.28% (W/V) NONYL-GLUCOSIDE, 0.5% (W/V) DECYL-GLUCOSIDE, 0.01% (W/V) DODECYL-GLUCOSIDE, 0.1% (W/V) CHOLESTERYLHEMISUCCINATE, 10MM HEPES PH 8, 1.15 MM NACL, 2 MM DTT, 100 NM NTI, 26% (V/V) PEG 600, 50 MM GLYCINE PH 9.4 |