3X1L

Crystal Structure of the CRISPR-Cas RNA Silencing Cmr Complex Bound to a Target Analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR system Cmr subunit Cmr2	polymer	677	78226.4	1	UniProt (Q8U1S6) Pfam (PF12469) Pfam (PF20823) Pfam (PF20822) Pfam (PF22335) Pfam (PF20824)	Pyrococcus furiosus DSM 3638	CRISPR-associated protein Cas10/Cmr2, subtype III-B
2	B (B)	CRISPR system Cmr subunit Cmr3	polymer	322	36366.4	1	UniProt (Q8U1S7) Pfam (PF09700)	Pyrococcus furiosus DSM 3638	CRISPR type III-B/RAMP module-associated protein Cmr3
3	C, D, E (C, D, E)	Cmr4	polymer	357	39865.8	3	UniProt (O28416) Pfam (PF03787)	Archaeoglobus fulgidus DSM 4304
4	F, G (F, G)	CRISPR system Cmr subunit Cmr5	polymer	155	17407.7	2	UniProt (O28417) Pfam (PF09701)	Archaeoglobus fulgidus DSM 4304	CRISPR type III-B/RAMP module-associated protein Cmr5
5	H (H)	Cmr6	polymer	349	40298.8	1	UniProt (O28418) Pfam (PF03787)	Archaeoglobus fulgidus DSM 4304
6	I (I)	RNA (32-MER)	polymer	39	12604.5	1	GenBank (CP003685)	Pyrococcus furiosus COM1
7	J (J)	DNA (5'-D(*TP*GP*CP*TP*CP*TP*CP*AP*GP*CP*CP*GP*CP*AP*AP*GP*GP*AP*CP*CP*GP*CP*AP*TP*AP*CP*TP*AP*CP*AP*A)-3')	polymer	31	9451.1	1		synthetic
8	K (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
9	L (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
10	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	130	Chemie (HOH)

Sequence modifications

A: 216 - 871 (UniProt: Q8U1S6)

PDB	External Database	Details
Met 195	-	expression tag
Gly 196	-	expression tag
Ser 197	-	expression tag
Ser 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
Ser 205	-	expression tag
Ser 206	-	expression tag
Gly 207	-	expression tag
Leu 208	-	expression tag
Val 209	-	expression tag
Pro 210	-	expression tag
Arg 211	-	expression tag
Gly 212	-	expression tag
Ser 213	-	expression tag
His 214	-	expression tag
Met 215	-	expression tag

C, D, E: 1 - 355 (UniProt: O28416)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag

H: 1 - 343 (UniProt: O28418)

PDB	External Database	Details
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	331360.0
Non-Polymers*	Number of molecules	2
	Total formula weight	89.7
All*	Total formula weight	331449.7

*Water molecules are not included.