3X1L
Crystal Structure of the CRISPR-Cas RNA Silencing Cmr Complex Bound to a Target Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.489, 76.215, 139.188 |
| Unit cell angles | 90.32, 104.83, 118.58 |
Refinement procedure
| Resolution | 44.338 - 2.096 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.627 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.096 | 2.100 |
| Number of reflections | 148845 | |
| Completeness [%] | 97.4 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 13-15% (w/v) PEG 3350, 100mM succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
