3WY0

The I375W mutant of CsyB complexed with CoA-SH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Putative uncharacterized protein csyB	polymer	417	45380.4	2	UniProt (Q53U84) Pfam (PF00195) Pfam (PF02797) UniProt (by SIFTS) (Q2U852) In PDB	Aspergillus oryzae (Yellow koji mold)	Type III polyketide synthase CsyB
2	A, B	COENZYME A	non-polymer		767.5	2	Chemie (COA)
3		water	water		18.0	655	Chemie (HOH)

Sequence modifications

A, B: 1 - 397 (UniProt: Q53U84)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Trp 375	Ile 375	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90760.8
Non-Polymers*	Number of molecules	2
	Total formula weight	1535.1
All*	Total formula weight	92295.8

*Water molecules are not included.