3WTP

Crystal Structure of the heterotypic nucleosome containing human CENP-A and H3.3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone H3-like centromeric protein A	polymer	143	16305.9	1	UniProt (P49450) Pfam (PF00125)	Homo sapiens (human)	Centromere autoantigen A, Centromere protein A, CENP-A
2	B, F (B, F)	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.2, Histone H2A/a, Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (human)	Histone H2B.1, Histone H2B.r, H2B/r
5	E (E)	Histone H3.3	polymer	140	15710.4	1	UniProt (P84243) Pfam (PF00125)	Homo sapiens (human)
6	I, J (I, J)	DNA (146-MER)	polymer	146	45053.9	2		Homo sapiens

Sequence modifications

A: 1 - 140 (UniProt: P49450)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

E: 0 - 135 (UniProt: P84243)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	202808.1
All*	Total formula weight	202808.1

*Water molecules are not included.