3WTP
Crystal Structure of the heterotypic nucleosome containing human CENP-A and H3.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97319 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.117, 107.667, 168.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.667 - 2.670 |
R-factor | 0.2306 |
Rwork | 0.229 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cv5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.005 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.790 |
High resolution limit [Å] | 2.670 | 5.340 | 2.670 |
Number of reflections | 50779 | ||
<I/σ(I)> | 9.8 | 24.8 | 2.7 |
Completeness [%] | 98.0 | 99 | 90 |
Redundancy | 5.5 | 6.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride, pH 6.0, vapor diffusion, hanging drop, temperature 293K |