3WNX

Crystal structure of ERGIC-53/MCFD2, Calcium/Man3-bound form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein ERGIC-53	polymer	246	27154.2	1	UniProt (P49257) Pfam (PF03388) In PDB	Homo sapiens (human)	ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1
2	B	Multiple coagulation factor deficiency protein 2	polymer	104	12056.2	1	UniProt (Q8NI22) Pfam (PF13499) In PDB	Homo sapiens (human)	MCFD2, Neural stem cell-derived neuronal survival protein
3	C	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose	branched		342.3	1	In PDB BIRD (PRD_900111) GlyTouCan (G53402KW)		2alpha-alpha-mannobiose
4	A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5		water	water		18.0	3	Chemie (HOH)

Sequence modifications

A: 31 - 269 (UniProt: P49257)

PDB	External Database	Details
Met 24	-	expression tag
Asn 25	-	expression tag
His 26	-	expression tag
Lys 27	-	expression tag
Val 28	-	expression tag
His 29	-	expression tag
Met 30	-	expression tag

B: 67 - 146 (UniProt: Q8NI22)

PDB	External Database	Details
Met 43	-	expression tag
Gly 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
His 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
Ser 55	-	expression tag
Ser 56	-	expression tag
Gly 57	-	expression tag
His 58	-	expression tag
Ile 59	-	expression tag
Glu 60	-	expression tag
Gly 61	-	expression tag
Arg 62	-	expression tag
His 63	-	expression tag
Met 64	-	expression tag
Leu 65	-	expression tag
Glu 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39210.4
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	39713.0

*Water molecules are not included.