3WNX
Crystal structure of ERGIC-53/MCFD2, Calcium/Man3-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.257, 58.212, 55.435 |
| Unit cell angles | 90.00, 109.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.750 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.28680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3whu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.079 | 0.438 |
| Number of reflections | 8017 | |
| <I/σ(I)> | 31.1 | 4 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% PEG5000 monomethyl ether, 100mM Bis-Tris, 10mM CaCl2, 10mM alpha2-mannotriose, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
