3WIE

Structure of a glucose dehydrogenase T277F mutant in complex with D-glucose and NAADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glucose 1-dehydrogenase	polymer	369	41414.1	4	UniProt (Q979W2) Pfam (PF08240) Pfam (PF16912)	Thermoplasma volcanium	GDH, GlcDH
2	E, F, I, J, M... (A, B, C, D)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
3	G, K, O, S (A, B, C, D)	[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4R,5R)-5-(3-carboxypyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate	non-polymer		744.4	4	Chemie (DN4)
4	H, L, P, T (A, B, C, D)	beta-D-glucopyranose	non-polymer		180.2	4	Chemie (BGC)
5	U, V, W, X (A, B, C, D)	water	water		18.0	216	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 361 (UniProt: Q979W2)

PDB	External Database	Details
Phe 277	Thr 277	engineered mutation
Leu 362	-	expression tag
Glu 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	165656.5
Non-Polymers*	Number of molecules	16
	Total formula weight	4221.5
All*	Total formula weight	169877.9

*Water molecules are not included.