3WID
Structure of a glucose dehydrogenase T277F mutant in complex with NADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glucose 1-dehydrogenase | polymer | 369 | 41414.1 | 4 | UniProt (Q979W2) Pfam (PF08240) Pfam (PF16912) | Thermoplasma volcanium | GDH, GlcDH |
| 2 | E, J, O, U (A, B, C, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | F, K, P, V (A, B, C, D) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) | |||
| 4 | G, L, Q, W (A, B, C, D) | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 4 | Chemie (PGO) | |||
| 5 | H, I, M, N, R... (A, B, C, D) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 8 | Chemie (1PE) | |||
| 6 | AA, BA, Y, Z (C, D, A, B) | water | water | 18.0 | 328 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 361 (UniProt: Q979W2)
| PDB | External Database | Details |
|---|---|---|
| Phe 277 | Thr 277 | engineered mutation |
| Leu 362 | - | expression tag |
| Glu 363 | - | expression tag |
| His 364 | - | expression tag |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 165656.5 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 5445.9 | |
| All* | Total formula weight | 171102.3 |
