3WDR

Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus complexed with gluco-manno-oligosaccharide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-mannanase	polymer	355	40256.7	1	UniProt (H7CGE2)	Symbiotic protist of Reticulitermes speratus
2	B (B)	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-mannopyranose	branched		666.6	1
3	C (C)	beta-D-glucopyranose-(1-4)-alpha-D-mannopyranose	branched		342.3	1
4	D, E, F (A)	beta-D-mannopyranose	non-polymer		180.2	3	Chemie (BMA)
5	G (A)	BICARBONATE ION	non-polymer		61.0	1	Chemie (BCT)
6	H, I (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7	J, K (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	L (A)	water	water		18.0	374	Chemie (HOH)

Sequence modifications

A: 15 - 344 (UniProt: H7CGE2)

PDB	External Database	Details
Glu -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Gln -7	-	expression tag
Lys -6	-	expression tag
Leu -5	-	expression tag
Ile -4	-	expression tag
Ser -3	-	expression tag
Glu -2	-	expression tag
Glu -1	-	expression tag
Asp 0	-	expression tag
Leu 1	-	expression tag
Asn 2	-	expression tag
Ser 3	-	expression tag
Ala 4	-	expression tag
Val 5	-	expression tag
Asp 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
Glu 13	-	expression tag
Phe 14	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40256.7
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	8
	Total formula weight	696.1
All*	Total formula weight	41961.7

*Water molecules are not included.