3WDR
Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus complexed with gluco-manno-oligosaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.522, 61.644, 58.068 |
Unit cell angles | 90.00, 92.27, 90.00 |
Refinement procedure
Resolution | 36.510 - 1.400 |
R-factor | 0.13845 |
Rwork | 0.137 |
R-free | 0.15983 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Substrate free structure of RsMan26C |
RMSD bond length | 0.011 |
RMSD bond angle | 1.537 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 62607 | |
<I/σ(I)> | 4.3 | |
Completeness [%] | 99.2 | 98.5 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG3350, 0.2M magnesium chloride, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |