3W3S

Crystal structure of A. aeolicus tRNASec in complex with M. kandleri SerRS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Type-2 serine--tRNA ligase	polymer	527	62155.4	1	UniProt (Q8TVD2) Pfam (PF18490) Pfam (PF00587) In PDB	Methanopyrus kandleri	Seryl-tRNA synthetase, SerRS, Seryl-tRNA(Ser/Sec) synthetase
2	B	selenocysteine tRNA	polymer	99	31897.9	1	GenBank (6626248)	Aquifex aeolicus VF5
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	5'-O-(N-(L-SERYL)-SULFAMOYL)ADENOSINE	non-polymer		433.4	1	Chemie (SSA)
5	A	PLATINUM (II) ION	non-polymer		195.1	15	Chemie (PT)
6	B, A	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)

Sequence modifications

A: 1 - 527 (UniProt: Q8TVD2)

PDB	External Database	Details
Tyr 55	Arg 55	ENGINEERED MUTATION
Tyr 58	Glu 58	ENGINEERED MUTATION
Tyr 62	Glu 62	ENGINEERED MUTATION
Tyr 116	Arg 116	ENGINEERED MUTATION
Tyr 118	Asp 118	ENGINEERED MUTATION
Arg 189	Glu 189	ENGINEERED MUTATION
Arg 193	Asp 193	ENGINEERED MUTATION
Tyr 379	Glu 379	ENGINEERED MUTATION
Arg 383	Glu 383	ENGINEERED MUTATION
Tyr 497	Glu 497	ENGINEERED MUTATION
Tyr 499	Glu 499	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	94053.3
Non-Polymers*	Number of molecules	25
	Total formula weight	4193.5
All*	Total formula weight	98246.8

*Water molecules are not included.