3W15

Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Peroxisomal targeting signal 2 receptor	polymer	368	41525.8	1	UniProt (P39108) Pfam (PF00400)	Saccharomyces cerevisiae (yeast)	PTS2 receptor, Peroxin-7, Peroxisome import protein PAS7
2	B (B)	Peroxisomal membrane protein PEX21	polymer	101	11004.1	1	UniProt (P50091)	Saccharomyces cerevisiae (yeast)	Peroxin-21
3	C (C)	3-ketoacyl-CoA thiolase, peroxisomal, Maltose-binding periplasmic protein	polymer	389	42897.6	1	UniProt (P27796) UniProt (P0AEX9) Pfam (PF01547)	Saccharomyces cerevisiae (yeast)	FOX3, MBP, MMBP, Maltodextrin-binding protein
4	D, E, G, H, I... (A, B, C)	NITRATE ION	non-polymer		62.0	9	Chemie (NO3)
5	F, N, O, P, Q... (A, C)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
6	S, T, U (A, B, C)	water	water		18.0	563	Chemie (HOH)

Sequence modifications

A: 1 - 375 (UniProt: P39108)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
-	Glu 257	deletion
-	Ser 258	deletion
-	Ala 259	deletion
-	Thr 260	deletion
-	Ile 261	deletion
-	Lys 262	deletion
-	Arg 263	deletion
-	Thr 264	deletion
-	Val 265	deletion

B: 190 - 288 (UniProt: P50091)

PDB	External Database	Details
Gly 188	-	expression tag
Ser 189	-	expression tag

C: 1 - 15 (UniProt: P27796)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 18 - 387 (UniProt: P0AEX9)

PDB	External Database	Details
Arg 16	-	linker
Ser 17	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95427.5
Non-Polymers*	Number of molecules	15
	Total formula weight	703.9
All*	Total formula weight	96131.4

*Water molecules are not included.