3W13

Insulin receptor ectodomain construct comprising domains L1-CR in complex with high-affinity insulin analogue [D-PRO-B26]-DTI-NH2, alphact peptide(693-719) and FAB 83-7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	Insulin receptor domains L1-CR	polymer	310	35503.9	1	UniProt (P06213) Pfam (PF01030) Pfam (PF00757) In PDB	Homo sapiens (human)	insulin receptor L1-CR (IR310.T), Insulin receptor subunit alpha
2	C	monoclonal antibody fab 83-7 fragment - heavy chain	polymer	116	12712.2	1		Mus musculus (mouse)
3	D	monoclonal antibody fab 83-7 fragment - light chain	polymer	114	12684.3	1		Mus musculus (mouse)
4	A	Insulin A chain	polymer	21	2383.7	1	UniProt (P01308) In PDB	HOMO SAPIENS
5	B	Insulin B chain	polymer	26	2938.4	1	UniProt (P01308) In PDB	HOMO SAPIENS
6	F	Insulin receptor alphact peptide	polymer	23	2788.1	1	UniProt (P06213) In PDB	HOMO SAPIENS	Insulin receptor subunit alpha
7	G	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
8	H	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
9	E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

E: 1 - 310 (UniProt: P06213)

PDB	External Database	Details
His 144	Tyr 171	SEE REMARK 999

B: 1 - 26 (UniProt: P01308)

PDB	External Database	Details
Pr9 26	Tyr 50	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	69010.6
Branched	Number of molecules	2
	Total formula weight	1335.2
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	70788.3

*Water molecules are not included.