3W0R

Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia (N202A), ternary complex with AMP-PNP and hygromycin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hygromycin-B 4-O-kinase	polymer	349	39033.8	1	UniProt (P00557) Pfam (PF01636) In PDB	Escherichia coli	APH(4), Hygromycin B phosphotransferase, Hygromycin-B kinase
2	A	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
3	A	HYGROMYCIN B VARIANT	non-polymer		527.5	1	Chemie (HY0)
4		water	water		18.0	147	Chemie (HOH)

Sequence modifications

A: 1 - 341 (UniProt: P00557)

PDB	External Database	Details
Gly 20	Asp 20	engineered mutation
Val 118	Ala 118	engineered mutation
Ala 202	Asn 202	engineered mutation
Pro 225	Ser 225	engineered mutation
Leu 226	Gln 226	engineered mutation
Ala 246	Thr 246	engineered mutation
Leu 342	-	expression tag
Glu 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39033.8
Non-Polymers*	Number of molecules	2
	Total formula weight	1033.7
All*	Total formula weight	40067.5

*Water molecules are not included.