3W0P
Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia (D198A), ternary complex with ADP and hygromycin B
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Hygromycin-B 4-O-kinase | polymer | 349 | 39032.8 | 1 | UniProt (P00557) Pfam (PF01636) In PDB | Escherichia coli | APH(4), Hygromycin B phosphotransferase, Hygromycin-B kinase |
2 | A | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
3 | A | HYGROMYCIN B VARIANT | non-polymer | 527.5 | 1 | Chemie (HY0) | |||
4 | water | water | 18.0 | 234 | Chemie (HOH) |
Sequence modifications
A: 1 - 341 (UniProt: P00557)
PDB | External Database | Details |
---|---|---|
Gly 20 | Asp 20 | engineered mutation |
Val 118 | Ala 118 | engineered mutation |
Ala 198 | Asp 198 | engineered mutation |
Pro 225 | Ser 225 | engineered mutation |
Leu 226 | Gln 226 | engineered mutation |
Ala 246 | Thr 246 | engineered mutation |
Leu 342 | - | expression tag |
Glu 343 | - | expression tag |
His 344 | - | expression tag |
His 345 | - | expression tag |
His 346 | - | expression tag |
His 347 | - | expression tag |
His 348 | - | expression tag |
His 349 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 39032.8 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 954.7 | |
All* | Total formula weight | 39987.5 |