3W0N
Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia, ternary complex with AMP-PNP and hygromycin B
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Hygromycin-B 4-O-kinase | polymer | 349 | 39076.8 | 1 | UniProt (P00557) Pfam (PF01636) | Escherichia coli | APH(4), Hygromycin B phosphotransferase, Hygromycin-B kinase |
| 2 | B (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 3 | C (A) | HYGROMYCIN B VARIANT | non-polymer | 527.5 | 1 | Chemie (HY0) | |||
| 4 | D (A) | water | water | 18.0 | 219 | Chemie (HOH) |
Sequence modifications
A: 1 - 341 (UniProt: P00557)
| PDB | External Database | Details |
|---|---|---|
| Gly 20 | Asp 20 | engineered mutation |
| Val 118 | Ala 118 | engineered mutation |
| Pro 225 | Ser 225 | engineered mutation |
| Leu 226 | Gln 226 | engineered mutation |
| Ala 246 | Thr 246 | engineered mutation |
| Leu 342 | - | expression tag |
| Glu 343 | - | expression tag |
| His 344 | - | expression tag |
| His 345 | - | expression tag |
| His 346 | - | expression tag |
| His 347 | - | expression tag |
| His 348 | - | expression tag |
| His 349 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 39076.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1033.7 | |
| All* | Total formula weight | 40110.5 |
