3VTW

Crystal structure of T7-tagged Optineurin LIR-fused human LC3B_2-119

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Optineurin, microtubule-associated proteins 1A/1B light chain 3B	polymer	149	17175.4	3	UniProt (Q96CV9) UniProt (Q9GZQ8) Pfam (PF02991)	Homo sapiens (human)	Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta
2	D, E, F, G (A, B, C)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	H, I, J (A, B, C)	water	water		18.0	85	Chemie (HOH)

Sequence modifications

A, B, C: 3 - 14 (UniProt: Q96CV9)

PDB	External Database	Details
Gly -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Met -11	-	expression tag
Ala -10	-	expression tag
Ser -9	-	expression tag
Met -8	-	expression tag
Thr -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Gln -4	-	expression tag
Gln -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Glu 3	Ser 170	engineered mutation
Glu 4	Ser 171	engineered mutation
Glu 6	Ser 173	engineered mutation
Glu 7	Ser 174	engineered mutation
Glu 10	Ser 177	engineered mutation
Gly 15	-	linker
Gly 16	-	linker

A, B, C: 17 - 134 (UniProt: Q9GZQ8)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	51526.2
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	51910.5

*Water molecules are not included.