3VHX

The crystal structure of Arf6-MKLP1 (Mitotic kinesin-like protein 1) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	ADP-ribosylation factor 6	polymer	172	19990.9	4	UniProt (P62331) Pfam (PF00025) In PDB	Mus musculus (mouse)
2	B, D, F, H	Kinesin-like protein KIF23	polymer	120	13515.3	4	UniProt (Q02241) Pfam (PF16540) In PDB	Homo sapiens (human)	Kinesin-like protein 5, Mitotic kinesin-like protein 1
3	A, C, E, G	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)
4	A, C, E, G	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	F	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	105	Chemie (HOH)

Sequence modifications

A, C, E, G: 13 - 175 (UniProt: P62331)

PDB	External Database	Details
Met 12	-	expression tag
Leu 67	Gln 67	engineered mutation
Leu 176	-	expression tag
Glu 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag

B, D, F, H: 690 - 807 (UniProt: Q02241)

PDB	External Database	Details
Gly 688	-	expression tag
Ser 689	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	134024.6
Non-Polymers*	Number of molecules	9
	Total formula weight	2282.0
All*	Total formula weight	136306.7

*Water molecules are not included.