3VHX

The crystal structure of Arf6-MKLP1 (Mitotic kinesin-like protein 1) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	ADP-ribosylation factor 6	polymer	172	19990.9	4	UniProt (P62331) Pfam (PF00025)	Mus musculus (mouse)
2	B, D, F, H (B, D, F, H)	Kinesin-like protein KIF23	polymer	120	13515.3	4	UniProt (Q02241) Pfam (PF16540)	Homo sapiens (human)	Kinesin-like protein 5, Mitotic kinesin-like protein 1
3	I, K, M, P (A, C, E, G)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	J, L, N, Q (A, C, E, G)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
5	O (F)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
6	R, S, T, U, V... (A, B, C, D, E...)	water	water		18.0	105	Chemie (HOH)

Sequence modifications

A, C, E, G: 13 - 175 (UniProt: P62331)

PDB	External Database	Details
Met 12	-	expression tag
Leu 67	Gln 67	engineered mutation
Leu 176	-	expression tag
Glu 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag

B, D, F, H: 690 - 807 (UniProt: Q02241)

PDB	External Database	Details
Gly 688	-	expression tag
Ser 689	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	134024.6
Non-Polymers*	Number of molecules	9
	Total formula weight	2282.0
All*	Total formula weight	136306.7

*Water molecules are not included.