Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3VHX

The crystal structure of Arf6-MKLP1 (Mitotic kinesin-like protein 1) complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001726	cellular_component	ruffle
A	0001889	biological_process	liver development
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005768	cellular_component	endosome
A	0005769	cellular_component	early endosome
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0005938	cellular_component	cell cortex
A	0006886	biological_process	intracellular protein transport
A	0007049	biological_process	cell cycle
A	0007399	biological_process	nervous system development
A	0010008	cellular_component	endosome membrane
A	0010975	biological_process	regulation of neuron projection development
A	0010976	biological_process	positive regulation of neuron projection development
A	0015031	biological_process	protein transport
A	0016020	cellular_component	membrane
A	0016192	biological_process	vesicle-mediated transport
A	0016787	molecular_function	hydrolase activity
A	0019003	molecular_function	GDP binding
A	0030139	cellular_component	endocytic vesicle
A	0030154	biological_process	cell differentiation
A	0030838	biological_process	positive regulation of actin filament polymerization
A	0030866	biological_process	cortical actin cytoskeleton organization
A	0031527	cellular_component	filopodium membrane
A	0031901	cellular_component	early endosome membrane
A	0031996	molecular_function	thioesterase binding
A	0032154	cellular_component	cleavage furrow
A	0032456	biological_process	endocytic recycling
A	0034143	biological_process	regulation of toll-like receptor 4 signaling pathway
A	0034394	biological_process	protein localization to cell surface
A	0035020	biological_process	regulation of Rac protein signal transduction
A	0035591	molecular_function	signaling adaptor activity
A	0036010	biological_process	protein localization to endosome
A	0042995	cellular_component	cell projection
A	0043209	cellular_component	myelin sheath
A	0048488	biological_process	synaptic vesicle endocytosis
A	0050714	biological_process	positive regulation of protein secretion
A	0051301	biological_process	cell division
A	0051489	biological_process	regulation of filopodium assembly
A	0051549	biological_process	positive regulation of keratinocyte migration
A	0055037	cellular_component	recycling endosome
A	0055038	cellular_component	recycling endosome membrane
A	0060998	biological_process	regulation of dendritic spine development
A	0072659	biological_process	protein localization to plasma membrane
A	0090162	biological_process	establishment of epithelial cell polarity
A	0090543	cellular_component	Flemming body
A	0097178	biological_process	ruffle assembly
A	0097284	biological_process	hepatocyte apoptotic process
A	0098793	cellular_component	presynapse
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099562	biological_process	maintenance of postsynaptic density structure
A	0120183	biological_process	positive regulation of focal adhesion disassembly
A	1902217	biological_process	erythrocyte apoptotic process
A	1903078	biological_process	positive regulation of protein localization to plasma membrane
A	1905606	biological_process	regulation of presynapse assembly
A	1990090	biological_process	cellular response to nerve growth factor stimulus
A	2000009	biological_process	negative regulation of protein localization to cell surface
A	2000171	biological_process	negative regulation of dendrite development
C	0000166	molecular_function	nucleotide binding
C	0001726	cellular_component	ruffle
C	0001889	biological_process	liver development
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005768	cellular_component	endosome
C	0005769	cellular_component	early endosome
C	0005794	cellular_component	Golgi apparatus
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0005938	cellular_component	cell cortex
C	0006886	biological_process	intracellular protein transport
C	0007049	biological_process	cell cycle
C	0007399	biological_process	nervous system development
C	0010008	cellular_component	endosome membrane
C	0010975	biological_process	regulation of neuron projection development
C	0010976	biological_process	positive regulation of neuron projection development
C	0015031	biological_process	protein transport
C	0016020	cellular_component	membrane
C	0016192	biological_process	vesicle-mediated transport
C	0016787	molecular_function	hydrolase activity
C	0019003	molecular_function	GDP binding
C	0030139	cellular_component	endocytic vesicle
C	0030154	biological_process	cell differentiation
C	0030838	biological_process	positive regulation of actin filament polymerization
C	0030866	biological_process	cortical actin cytoskeleton organization
C	0031527	cellular_component	filopodium membrane
C	0031901	cellular_component	early endosome membrane
C	0031996	molecular_function	thioesterase binding
C	0032154	cellular_component	cleavage furrow
C	0032456	biological_process	endocytic recycling
C	0034143	biological_process	regulation of toll-like receptor 4 signaling pathway
C	0034394	biological_process	protein localization to cell surface
C	0035020	biological_process	regulation of Rac protein signal transduction
C	0035591	molecular_function	signaling adaptor activity
C	0036010	biological_process	protein localization to endosome
C	0042995	cellular_component	cell projection
C	0043209	cellular_component	myelin sheath
C	0048488	biological_process	synaptic vesicle endocytosis
C	0050714	biological_process	positive regulation of protein secretion
C	0051301	biological_process	cell division
C	0051489	biological_process	regulation of filopodium assembly
C	0051549	biological_process	positive regulation of keratinocyte migration
C	0055037	cellular_component	recycling endosome
C	0055038	cellular_component	recycling endosome membrane
C	0060998	biological_process	regulation of dendritic spine development
C	0072659	biological_process	protein localization to plasma membrane
C	0090162	biological_process	establishment of epithelial cell polarity
C	0090543	cellular_component	Flemming body
C	0097178	biological_process	ruffle assembly
C	0097284	biological_process	hepatocyte apoptotic process
C	0098793	cellular_component	presynapse
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099562	biological_process	maintenance of postsynaptic density structure
C	0120183	biological_process	positive regulation of focal adhesion disassembly
C	1902217	biological_process	erythrocyte apoptotic process
C	1903078	biological_process	positive regulation of protein localization to plasma membrane
C	1905606	biological_process	regulation of presynapse assembly
C	1990090	biological_process	cellular response to nerve growth factor stimulus
C	2000009	biological_process	negative regulation of protein localization to cell surface
C	2000171	biological_process	negative regulation of dendrite development
E	0000166	molecular_function	nucleotide binding
E	0001726	cellular_component	ruffle
E	0001889	biological_process	liver development
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005769	cellular_component	early endosome
E	0005794	cellular_component	Golgi apparatus
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0006886	biological_process	intracellular protein transport
E	0007049	biological_process	cell cycle
E	0007399	biological_process	nervous system development
E	0010008	cellular_component	endosome membrane
E	0010975	biological_process	regulation of neuron projection development
E	0010976	biological_process	positive regulation of neuron projection development
E	0015031	biological_process	protein transport
E	0016020	cellular_component	membrane
E	0016192	biological_process	vesicle-mediated transport
E	0016787	molecular_function	hydrolase activity
E	0019003	molecular_function	GDP binding
E	0030139	cellular_component	endocytic vesicle
E	0030154	biological_process	cell differentiation
E	0030838	biological_process	positive regulation of actin filament polymerization
E	0030866	biological_process	cortical actin cytoskeleton organization
E	0031527	cellular_component	filopodium membrane
E	0031901	cellular_component	early endosome membrane
E	0031996	molecular_function	thioesterase binding
E	0032154	cellular_component	cleavage furrow
E	0032456	biological_process	endocytic recycling
E	0034143	biological_process	regulation of toll-like receptor 4 signaling pathway
E	0034394	biological_process	protein localization to cell surface
E	0035020	biological_process	regulation of Rac protein signal transduction
E	0035591	molecular_function	signaling adaptor activity
E	0036010	biological_process	protein localization to endosome
E	0042995	cellular_component	cell projection
E	0043209	cellular_component	myelin sheath
E	0048488	biological_process	synaptic vesicle endocytosis
E	0050714	biological_process	positive regulation of protein secretion
E	0051301	biological_process	cell division
E	0051489	biological_process	regulation of filopodium assembly
E	0051549	biological_process	positive regulation of keratinocyte migration
E	0055037	cellular_component	recycling endosome
E	0055038	cellular_component	recycling endosome membrane
E	0060998	biological_process	regulation of dendritic spine development
E	0072659	biological_process	protein localization to plasma membrane
E	0090162	biological_process	establishment of epithelial cell polarity
E	0090543	cellular_component	Flemming body
E	0097178	biological_process	ruffle assembly
E	0097284	biological_process	hepatocyte apoptotic process
E	0098793	cellular_component	presynapse
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0099562	biological_process	maintenance of postsynaptic density structure
E	0120183	biological_process	positive regulation of focal adhesion disassembly
E	1902217	biological_process	erythrocyte apoptotic process
E	1903078	biological_process	positive regulation of protein localization to plasma membrane
E	1905606	biological_process	regulation of presynapse assembly
E	1990090	biological_process	cellular response to nerve growth factor stimulus
E	2000009	biological_process	negative regulation of protein localization to cell surface
E	2000171	biological_process	negative regulation of dendrite development
G	0000166	molecular_function	nucleotide binding
G	0001726	cellular_component	ruffle
G	0001889	biological_process	liver development
G	0003924	molecular_function	GTPase activity
G	0003925	molecular_function	G protein activity
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005737	cellular_component	cytoplasm
G	0005768	cellular_component	endosome
G	0005769	cellular_component	early endosome
G	0005794	cellular_component	Golgi apparatus
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0005925	cellular_component	focal adhesion
G	0005938	cellular_component	cell cortex
G	0006886	biological_process	intracellular protein transport
G	0007049	biological_process	cell cycle
G	0007399	biological_process	nervous system development
G	0010008	cellular_component	endosome membrane
G	0010975	biological_process	regulation of neuron projection development
G	0010976	biological_process	positive regulation of neuron projection development
G	0015031	biological_process	protein transport
G	0016020	cellular_component	membrane
G	0016192	biological_process	vesicle-mediated transport
G	0016787	molecular_function	hydrolase activity
G	0019003	molecular_function	GDP binding
G	0030139	cellular_component	endocytic vesicle
G	0030154	biological_process	cell differentiation
G	0030838	biological_process	positive regulation of actin filament polymerization
G	0030866	biological_process	cortical actin cytoskeleton organization
G	0031527	cellular_component	filopodium membrane
G	0031901	cellular_component	early endosome membrane
G	0031996	molecular_function	thioesterase binding
G	0032154	cellular_component	cleavage furrow
G	0032456	biological_process	endocytic recycling
G	0034143	biological_process	regulation of toll-like receptor 4 signaling pathway
G	0034394	biological_process	protein localization to cell surface
G	0035020	biological_process	regulation of Rac protein signal transduction
G	0035591	molecular_function	signaling adaptor activity
G	0036010	biological_process	protein localization to endosome
G	0042995	cellular_component	cell projection
G	0043209	cellular_component	myelin sheath
G	0048488	biological_process	synaptic vesicle endocytosis
G	0050714	biological_process	positive regulation of protein secretion
G	0051301	biological_process	cell division
G	0051489	biological_process	regulation of filopodium assembly
G	0051549	biological_process	positive regulation of keratinocyte migration
G	0055037	cellular_component	recycling endosome
G	0055038	cellular_component	recycling endosome membrane
G	0060998	biological_process	regulation of dendritic spine development
G	0072659	biological_process	protein localization to plasma membrane
G	0090162	biological_process	establishment of epithelial cell polarity
G	0090543	cellular_component	Flemming body
G	0097178	biological_process	ruffle assembly
G	0097284	biological_process	hepatocyte apoptotic process
G	0098793	cellular_component	presynapse
G	0098794	cellular_component	postsynapse
G	0098978	cellular_component	glutamatergic synapse
G	0099562	biological_process	maintenance of postsynaptic density structure
G	0120183	biological_process	positive regulation of focal adhesion disassembly
G	1902217	biological_process	erythrocyte apoptotic process
G	1903078	biological_process	positive regulation of protein localization to plasma membrane
G	1905606	biological_process	regulation of presynapse assembly
G	1990090	biological_process	cellular response to nerve growth factor stimulus
G	2000009	biological_process	negative regulation of protein localization to cell surface
G	2000171	biological_process	negative regulation of dendrite development

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GTP A 184

Chain	Residue
A	ASP22
A	GLY66
A	ASN122
A	LYS123
A	ASP125
A	LEU126
A	CYS155
A	ALA156
A	THR157
A	MG185
A	HOH186
A	ALA23
A	HOH193
A	ALA24
A	GLY25
A	LYS26
A	THR27
A	THR28
A	THR41
A	THR44

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 185

Chain	Residue
A	THR27
A	THR44
A	GTP184
A	HOH193

site_id	AC3
Number of Residues	23
Details	BINDING SITE FOR RESIDUE GTP C 184

Chain	Residue
C	ASP22
C	ALA23
C	ALA24
C	GLY25
C	LYS26
C	THR27
C	THR28
C	THR41
C	PRO43
C	THR44
C	GLY65
C	GLY66
C	ASN122
C	LYS123
C	ASP125
C	LEU126
C	CYS155
C	ALA156
C	MG185
C	HOH204
C	HOH210
C	HOH211
C	HOH212

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 185

Chain	Residue
C	THR27
C	THR44
C	GTP184
C	HOH210
C	HOH211

site_id	AC5
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GTP E 184

Chain	Residue
E	ASP22
E	ALA23
E	ALA24
E	GLY25
E	LYS26
E	THR27
E	THR28
E	THR41
E	PRO43
E	THR44
E	GLY66
E	LEU67
E	ASN122
E	LYS123
E	ASP125
E	LEU126
E	CYS155
E	ALA156
E	MG185
E	HOH201
E	HOH204

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E 185

Chain	Residue
E	THR27
E	THR44
E	GTP184
E	HOH201
E	HOH204

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL F 1

Chain	Residue
E	ARG75
E	HIS76
F	PRO720
F	ALA721
F	SER722

site_id	AC8
Number of Residues	17
Details	BINDING SITE FOR RESIDUE GTP G 184

Chain	Residue
G	MG185
G	ASP22
G	ALA23
G	GLY25
G	LYS26
G	THR27
G	THR28
G	THR41
G	THR44
G	GLY66
G	ASN122
G	LYS123
G	ASP125
G	LEU126
G	CYS155
G	ALA156
G	THR157

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG G 185

Chain	Residue
G	THR27
G	THR44
G	GTP184

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17081983

Chain	Residue	Details
G	ASN122
B	SER710
D	SER710
F	SER710
H	SER710
C	ASP63
C	ASN122
E	ALA23
E	ASP63
E	ASN122
G	ALA23
G	ASP63

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
D	SER763
G	CYS155
B	SER763
F	SER763
H	SER763
E	THR41
E	CYS155
G	THR41

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
B	SER798
D	SER798
F	SER798
H	SER798

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
B	SER807
D	SER807
F	SER807
H	SER807

site_id	SWS_FT_FI5
Number of Residues	20
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
B	LYS719
B	LYS750
B	LYS770
B	LYS795
D	LYS719
D	LYS750
D	LYS770
D	LYS795
F	LYS719
F	LYS750
F	LYS770
F	LYS795
H	LYS719
H	LYS750
H	LYS770
H	LYS795

site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:25755297, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
B	LYS773
D	LYS773
F	LYS773
H	LYS773

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)