3VH4

Crystal structure of Atg7CTD-Atg8-MgATP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-like modifier-activating enzyme ATG7	polymer	340	38150.9	1	UniProt (P38862) Pfam (PF00899) In PDB	Saccharomyces cerevisiae (Baker's yeast)	ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Cytoplasm to vacuole targeting protein 2
2	B	Autophagy-related protein 8	polymer	119	13751.9	1	UniProt (P38182) Pfam (PF02991) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 295 - 630 (UniProt: P38862)

PDB	External Database	Details
Gly 291	-	expression tag
Pro 292	-	expression tag
His 293	-	expression tag
Met 294	-	expression tag

B: 1 - 116 (UniProt: P38182)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Pro 26	Lys 26	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51902.7
Non-Polymers*	Number of molecules	3
	Total formula weight	596.9
All*	Total formula weight	52499.6

*Water molecules are not included.