3VD4

E. coli (lacZ) beta-galactosidase (N460D) in complex with IPTG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Beta-galactosidase	polymer	1052	119744.7	4	UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) In PDB	Escherichia coli	Beta-gal, Lactase
2	C, D, B, A	MAGNESIUM ION	non-polymer		24.3	9	Chemie (MG)
3	C, D, B, A	SODIUM ION	non-polymer		23.0	14	Chemie (NA)
4	A, D, C, B	DIMETHYL SULFOXIDE	non-polymer		78.1	68	Chemie (DMS)
5	D, B, C, A	1-methylethyl 1-thio-beta-D-galactopyranoside	non-polymer		238.3	4	Chemie (IPT)
6		water	water		18.0	3300	Chemie (HOH)

Sequence modifications

A, B, C, D: 9 - 1023 (UniProt: P00722)

PDB	External Database	Details
Met -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Gly -18	-	expression tag
Met -17	-	expression tag
Ala -16	-	expression tag
Ser -15	-	expression tag
Met -14	-	expression tag
Thr -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Gln -10	-	expression tag
Gln -9	-	expression tag
Met -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Asp -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Asp 1	-	expression tag
Lys 2	-	expression tag
Asp 3	-	expression tag
Pro 4	-	expression tag
Met 5	-	expression tag
Ile 6	-	expression tag
Asp 7	-	expression tag
Pro 8	-	expression tag
Asp 460	Asn 461	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	478979.0
Non-Polymers*	Number of molecules	95
	Total formula weight	6806.9
All*	Total formula weight	485785.8

*Water molecules are not included.