3VD4
E. coli (lacZ) beta-galactosidase (N460D) in complex with IPTG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.762, 162.446, 203.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.900 - 2.000 |
| R-factor | 0.17269 |
| Rwork | 0.172 |
| R-free | 0.21199 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jyx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 204.120 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.371 |
| Number of reflections | 312850 | |
| <I/σ(I)> | 10.5 | 2.5 |
| Completeness [%] | 93.0 | 67.1 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 10% PEG8000, 100 mM Bis-Tris, pH 6.5, 200 mM magnesium chloride, 100 mM sodium chloride, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
