3VAK

Structure of U2AF65 variant with BrU5 DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Splicing factor U2AF 65 kDa subunit	polymer	174	19075.8	2	UniProt (P26368) Pfam (PF00076)	Homo sapiens (human)	U2 auxiliary factor 65 kDa subunit, hU2AF(65), hU2AF65, U2 snRNP auxiliary factor large subunit
2	C, D (P, E)	DNA (5'-D(*UP*UP*UP*UP*(BRU)P*UP*U)-3')	polymer	7	2065.1	2		synthetic construct
3	E, F, N, O, P (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	5	Chemie (DIO)
4	G, H, I, J, R... (A, B)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
5	K, L, M, U (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL) PubChem (753)
6	Q (B)	N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE	non-polymer		862.1	1	Chemie (CPQ) PubChem (44658112) PubChem (45933557) PubChem (46936249) PubChem (16402218) PubChem (446320) PubChem (75361254) PubChem (90277687) PubChem (102565423) PubChem (124867695) PubChem (124905380) PubChem (124905378) PubChem (124919192) PubChem (124933353) PubChem (124933355) PubChem (125027417) PubChem (129891641) PubChem (132470929) PubChem (71299811) PubChem (135639079) PubChem (139981181) PubChem (122130317) PubChem (3609263) PubChem (124837856) PubChem (124905379) PubChem (124905381) PubChem (124919193) PubChem (124919194) PubChem (124919195) PubChem (124933354) PubChem (124933356) PubChem (171036883)
7	V, W, X, Y (A, B, P, E)	water	water		18.0	272	Chemie (HOH)

Sequence modifications

A, B: 148 - 237 (UniProt: P26368)

PDB	External Database	Details
Gly 143	-	expression tag
Pro 144	-	expression tag
Leu 145	-	expression tag
Gly 146	-	expression tag
Ser 147	-	expression tag

A, B: 258 - 336 (UniProt: P26368)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	42281.7
Non-Polymers*	Number of molecules	17
	Total formula weight	2343.4
All*	Total formula weight	44625.1

*Water molecules are not included.