3VAK

Structure of U2AF65 variant with BrU5 DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Splicing factor U2AF 65 kDa subunit	polymer	174	19075.8	2	UniProt (P26368) Pfam (PF00076) In PDB	Homo sapiens (human)	U2 auxiliary factor 65 kDa subunit, hU2AF(65), hU2AF65, U2 snRNP auxiliary factor large subunit
2	P, E	DNA (5'-D(*UP*UP*UP*UP*(BRU)P*UP*U)-3')	polymer	7	2065.1	2		synthetic construct
3	A, B	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	5	Chemie (DIO)
4	A, B	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
5	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
6	B	N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE	non-polymer		862.1	1	Chemie (CPQ)
7		water	water		18.0	272	Chemie (HOH)

Sequence modifications

A, B: 148 - 237 (UniProt: P26368)

PDB	External Database	Details
Gly 143	-	EXPRESSION TAG
Pro 144	-	EXPRESSION TAG
Leu 145	-	EXPRESSION TAG
Gly 146	-	EXPRESSION TAG
Ser 147	-	EXPRESSION TAG

A, B: 258 - 336 (UniProt: P26368)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	42281.7
Non-Polymers*	Number of molecules	17
	Total formula weight	2343.4
All*	Total formula weight	44625.1

*Water molecules are not included.